in Mcl-1L expression could be related to the differences in its stability or half-life across the cell types. In line with our previous report24, EtOH exposure caused a dramatic reduction in SRSF1 protein levels in hNSPs, hNPCs, and immature neurons. Interestingly, SRSF1 levels in mature neuron cultures were slightly reduced but maintained in cells exposed to EtOH suggesting that its expression levels were sufficient to maintain Mcl-1L splicing over Mcl-1S. The expression levels of other members of Bcl-2-associated genes, including Bcl-2, Bax, Bad, and Puma were not significantly altered by EtOH exposure in all the cells types analyzed.
