The two-bottle choice protocol was carried out as previously described (Blednov et al., 2001). Tubes were weighed every other day. Food was available ad libitum, and mice were weighed every 4 days. Tube positions were changed daily to control for position preferences. Ethanol consumption (g/kg body weight/24 hr) was calculated for each mouse and values summarized for every concentrations of ethanol. The range of ethanol concentrations was varied from % to 18%.
