An additional 424 genes were nominated based on behavioral genetic analysis of 255 measures of addiction related phenotypes obtained by the Tennessee Mouse Genome Consortium in the recently expanded panel of over 60 BXD recombinant inbred mouse lines (Chesler and colleagues, submitted). Because we are interested in identifying addiction associated pathway members that are polymorphic in humans, but not necessarily polymorphic in mice, our approach identified both candidate genes for mouse phenotypic variation, and biomolecular correlates of the mouse phenotypes. Each gene was chosen because it either resided in a significant or suggestive QTL interval or was a gene expression correlate of multiple addiction-related phenotypes. The criteria used to place genes on the list included correlations with p<.0001, and QTLs, with genome-wide permutation p<.05 or p<.33, the conventional thresholds for significant and suggestive loci. Convergence of evidence of these effects in multiple addiction assays was the criteria used for list membership. Mouse genes were then mapped to human genes via the HomoloGene database (http://www.ncbi.nlm.nih.gov/sites/entrez?db=homologene).
