Informed consent was obtained from all patients sequenced in this study. Peripheral blood mononuclear cells were isolated from patient donors, Ficoll separated, and cryopreserved by the UCSF Core Immunologic Laboratory (CIL). PBMCs were thawed in a 37°C water bath, and subsequently washed and resuspended in EasySep buffer (STEMCELL Technologies). Cells were treated with DNAseI and incubated for 15 min at RT before filtering through a 40um column. Finally, the cells were washed in EasySep and resuspended in 1x PBMS and 0.04% bovine serum albumin. Cells from 8 donors were then re-concentrated to 1M cells per mL and then serially pooled. At each pooling stage, 1M cells per mL were combined to result in a final sample pool with cells from all donors.
