Chunk #29 — MATERIALS AND METHODS — NPC generation from iPSCs by RA treatment

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Early pathogenic event of Alzheimer's disease documented in iPSCs from patients with PSEN1 mutations.

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The human ESC medium consisted of DMEM/F12 medium with 20% KSR, 1% GlutaMax-1, 1% non-essential amino acid (NEAA), 50 μM β-mercaptoethanol (ME), and 1% penicillin/streptomycin (P/S). The NSP medium consisted of DMEM/F12 medium containing 1% N2 supplement, 1% GlutaMax-1, 1% NEAA, 50 μM β-ME, 1% P/S, 8 μg/ml Heparin, 20 ng/ml bFGF, and 20 ng/ml Epidermal growth factor (EGF).