Many of these DNAm changes were further confirmed using WGBS data comparing one fetal sample to two adult NeuN+ and NeuN− samples from the study of Lister et al16 (see Supplementary Text 2) at all three spatial scales. Using Epigenome Roadmap data, many of the DNAm changes at birth were further associated with enhancer and repressive chromatin states in the adult DLPFC and suggest vastly different epigenetic landscapes of the prenatal versus postnatal human brain, consistent with our DNAm data (Supplementary Table 6, Supplementary Text 3). By matching these DNAm samples to publicly available gene expression data from the same donor, we confirmed that many of these DNA methylation changes associated with nearby gene expression levels, including at the CpG (71.0%, Supplementary Figure 3), DMR (87.3%, Supplementary Figure 4) and block (73.3%) spatial scales (Supplementary Text 4). These data, therefore, suggest a functional role for many of these DNA methylation changes in the developing human brain.
