Since neurons are susceptible to shear stress, we also wanted to ensure that the perfusions were well tolerated by the neurons. To address this, we immunostained for MAP2 following local perfusion with normal media (Figure 4F). After the perfusion, there was no detectable damage to dendritic morphology of the perfused segments. Together, these data show that we can deliver pharmacological agents to focal synaptic regions with quick response time and with minimal impact on dendritic structure using the μLP chamber.
