Cells were cultured in appropriate media and 40 μl was transferred into each well of a 384-well clear bottom, tissue culture treated plate with an automatic liquid handler. Plates were incubated at 37°C, 5% CO2. Cells were either treated with chemical or genetic perturbations, the details of which are reported in section 6 below. For cell lysis, media was removed from the wells without disturbing the cells and 25 μl/well of TCL Lysis Buffer (Qiagen) was added. Plates were sealed with adherent foil seals and incubated at room temperature for 30 minutes prior to storage at -80°C.
