Floating sections were processed for GFP fluorescent immunostaining. To localize the GFP-tagged lentivirus-infected cells in mice, we utilized a rabbit polyclonal IgG that recognizes GFP cloned from copepod Pontellina plumata (copGFP). To localize the lentivirus tagged with GFP in rats, we utilized a chicken polyclonal IgG that recognizes a 27kDa protein derived from the jellyfish Aequorea Victoria. Further, to identify IPN we utilized a guinea pig polyclonal IgG that recognizes VAChT. Sections were rinsed in 0.1M PBS, pH 7.4, with 0.3% Triton-X 100 (PBT) and then blocked in 10% normal donkey serum/PBT. Thereafter, sections were incubated in the primary antibody in PBT at 4°C overnight. The primary antibodies were diluted as follows: rabbit anti-copGFP (1:2,000; Evrogen, Moscow, Russia), chicken anti-GFP (1:2,000; Millipore, Billercia, MA) or guinea pig anti-VACHT (1:500; Millipore). On day 2, the sections were rinsed and incubated in Alexa 488 donkey anti-rabbit (1:400; Invitrogen), DyLight 488 donkey anti-chicken (1:400; Jackson ImmunoResearch, West Grove, PA) and/or DyLight 594 or 647 donkey anti-guinea pig (1:500; Jackson ImmunoResearch) secondary antibodies in 0.3% PBT for 2 hrs. Next, the sections were rinsed,
