cycle of 95°C for 0.25 min, 60°C for 0.25 min, and 95°C for 0.25 min, and was used to degrade short fragment primer dimers. The linear range for each sample was identified using SDS v2.1 software. Using the determined linear range, each sample was analyzed in duplicate by PCR using the FAM-labeled primer for PPP3CA_EX1. Products were resolved using an ABI 3100 sequence analyzer, as described. Peak area measurements were averaged. Relative peak areas for each allele were compared to produce an assessment of relative peak areas for each heterozygous sample for brain cDNAs and heterozygote genomic DNA standards.
