While single-cell sequencing technologies are capable of measuring RNA transcripts and surface proteins in thousands of single cells, cytometry-based techniques can measure both extracellular and intracellular proteins in millions of cells. As our bridge integration procedure should enable the mapping of CyTOF profiles onto scRNA-seq datasets, we obtained a collection of CyTOF datasets spanning 119 individuals and 5,170,249 total cells65. We used our previously collected CITE-seq dataset of 161,764 PBMC from healthy donors as a multi-omic bridge4. The CyTOF and CITE-seq dataset both shared 30 cell surface protein features, while the CyTOF dataset also measured 17 unique proteins which included intracellular targets that cannot be measured via CITE-seq.
