field stimulation, the stimulation paradigm was set using a Master-8 (A.M.P.I.). 30mA pulses were generated with a stimulus isolator (World Precision Instruments) connected to two platinum electrodes placed above the region being imaged. For analysis, regions of interest (ROIs) were placed on somas and neurites based on differences between z-stack maximum and minimum projections using Image J. Duration of the calcium events and traces were subsequently determined using “Event Detection Analysis” (EvA) with in-house Matlab software scripts as described previously [27]. Drug concentrations used are listed in Table C in S1 File.
