Demuxlet: demultiplexing and doublet identification from single cell dataa) Pipeline for experimental multiplexing of unrelated individuals, loading onto droplet-based single-cell RNA-sequencing instrument, and computational demultiplexing (demux) and doublet removal using demuxlet. Assuming equal mixing of 8 individuals, b) 4 genetic variants can recover the sample identity of a cell, and c) 87.5% of doublets will contain cells from two different samples.
