Chunk #67 — Materials and Methods — PCR Genotyping Procedures

Source

Essential role of chromatin remodeling protein Bptf in early mouse embryos and embryonic stem cells.

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BptfXG023 disruption was confirmed by 5′ RACE according to established procedures from Bptf/BptfXG023 total testis RNA according to procedures established by the Bay Genomics Gene Trap Consortium (http://baygenomics.ucsf.edu/). Two separate 5′ RACE reactions were sequenced giving identical results.