with MDD in the MEIS2, PAX6, and PAX5 regions (pval= 2.04×10−8, 3.94×10−7, and 2.59×10−5 in the 23andMe Discovery dataset). Tissue enrichment analysis showed an over-representation of central nervous system, with 12 of the 19 nominally associated tissues being from different brain regions (with a Nervous System as a second level MeSH term). Although these associations did not pass multiple-testing correction, the top results from our MDD GWAS are enriched for CNS expression and transcriptional function important for CNS development or neurogenesis. Further functional annotations of predicted genomic/molecular function, brain tissue or monocyte eQTLs, gene predictions for each region using DEPICT18, and disease associations using publicly available GWAS datasets and the OMIM database are presented in Supplementary Table 4 for all 17 SNPs reaching genome-wide significance in the joint-analysis (Table 2).
