Chunk #51 — Methods — TUNEL staining of apoptotic cells in mouse hippocampus

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Neurotoxic reactive astrocytes are induced by activated microglia.

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Neuronal cell death was detected in vivo in wildtype and single knock-out animals (Il1α−/−, TNFα−/−, or C1q−/−) using TUNEL staining of 12 μm 4% paraformaldehyde fixed frozen sections of hippocampus using the in situ cell death detection kit, TMR red (Roche, 12156792910) using supplier protocol.