as G9a), etc (Fig. 2a)22,23. That H3K9 but not H3K27 methylation is mentioned for regulation of OPC differentiation by Liu et al.24. If OLIG2 inhibits gene expression through H3K9 methylation to promote myelination, then disrupting H3K9 methylation will also affect the myelination. Knockdown experiments (Supplementary Fig. 2b, c) was performed in the primary rat OPCs to identify the most important enzymes for myelination. Silencing SETDB1, but not other epigenetic factors, not only led to markedly decreased expression of Mag and Mbp, makers of myelin (Fig. 2c), but also significantly impeded the efficacy of oligodendrocyte generation (Fig. 2d).
