Immunostainings were visualized on an inverted Leica confocal microscope (Leica TCS SPE-II with DMI4000 RYBC Leica TCS SPE confocal unit RYBV) equipped with 10×/0.30 CS, 40×/1.15 Oil CS and 63×/1.30 Oil CS objectives and appropriate filter sets (excitation: 405nm/25 mW, 488nm/10 mW, 561nm/20 mW). Phase-contrast images were taken using an inverted EVOS XL Imagin6g System (# AME3300) equipped with long working distance phase contrast objectives and LED illumination. Images were acquired with a digital 3 MP CMOS color camera.
