Table 14. Ct values were averaged from two technical replicates for each gene. Geometric mean of average Ct for the housekeeping genes GAPDH, B2M and ACTB was used as a reference that was subtracted from the average Ct for a gene of interest (dCt). Gene expression levels were log transformed (2−dCt) and related to the combined mean values of pcDNA3 and pGFP-V-RS-shSCR control samples in each sort giving relative expression for each gene of interest. Data were visualized in GraphPad Prism 7 (GraphPad Software). Four independent experiments were performed without randomization of sample processing, n = 4. Researcher was not blinded to the sample identity. Differences between means were analyzed using one-way ANOVA and Dunnett’s post hoc multiple comparisons test between experimental and control groups, with a single pooled variance. Adjusted P values for each comparison are reported in Supplementary Table 13.
