To increase the utility of CBV-based layer-fMRI acquisition and analysis methods for the application in human cognitive neuroscience, all of the above limitations must be addressed. Only, then these methods can be used in more sophisticated task designs, in higher-order brain areas, and while capturing subtle changes in brain networks. In this article, we argue that with the methodological advancements in the past years, the aforementioned limitations are largely resolved. Layer-fMRI connectivity measures are now available without venous contamination and with whole-brain coverage. This paves the road for a new class of neuroscience questions to be investigated. We aim to exemplify how layer-dependent whole-brain fMRI datasets might be used for a novel family of connectivity analyses by discussing multiple examples of layer-dependent connectivity analysis procedures that will shed new light into the internal working principles and functional organization of the human brain. In this paper, we show several examples of layer-specific fMRI.
