To investigate further the validity of these results, we performed mixed model analyses in SPSS on the extracted gray matter volumes for the middle cingulum and middle temporal cortex. This analysis revealed a reduction in the volume of the right middle cingulum (F=4.49, df =1, 32, p = 0.042) for the prenatally exposed group relative to the non-exposed group (Figure 9), though no differences were found in the left middle temporal region (Table 5a). As may be seen (Figure 9), the right middle cingulum showed reduction in volume in association with prenatal alcohol exposure.