Chunk #66 — STAR★METHODS — QUANTIFICATION AND STATISTICAL ANALYSIS — FISH image quantification

Source

Transcriptional and anatomical diversity of medium spiny neurons in the primate striatum.

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except that we drew ROIs for the whole CPNE4 and RXFP1 clusters after separating overlapping nuclei with the “Watershed” function. As control, we drew ROIs of roughly similar size to the RXFP1 or CPNE4 clusters in nearby regions and quantified the cells expressing DRD1.