Plasma FGF21 and insulin, respectively, were assayed using the rat/mouse FGF21 ELISA kit (EMD Millipore) and the ultrasensitive mouse insulin ELISA kit (Crystal Chem) following the manufacturer's instructions. Aspartate transaminase, alanine transaminase (ALT), total cholesterol, LDL cholesterol and HDL cholesterol were determined using a COBAS-MIRA+ biochemical analyser (Anexplo facility).
