Caspase-1 activity was measured by the Colorimetric Assay Kit (Abcam). This assay allows the detection of chromophore p-nitroanilide (pNA) after cleavage from labeled substrate YVAD-pNA, performed following the manufacturer’s instructions. pNA light emission was quantified by a spectrophotometer at 405 nm. The comparison of the absorbance of pNA from treated vs. untreated controls allowed the determination of the fold increase in caspase-1 activity.
