amplification was carried out in a final volume of 30 μl consisting of 50 ng genomic DNA, 2.5 mM dNTPs, 0.1 μg of sense and antisense primers, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2, 5% dimethyl sulfoxide, and 1 U of Taq DNA polymerase. The reaction was then carried out with an initial denaturation for 2 min at 95°C, followed by 35 cycles of 95°C, 1 min/61°C, 1 min/72°C, and a final extension at 72° C for 7 minutes. Allele calls were verified by independent assessment from two investigators.
