and neurons largely grouped in gene expression space by Cit status; however, some Cit− cells mapped with neurons and vice versa, and a small number of cells (~8.3%) mapped to an intermediate differentiation stage. Similarly, neurons and progenitors both grouped predominately based on number of days in culture. Of note, not much separation was detected between D100 and D125 cells, which could be an indication that transcriptional changes do not underlie late-stage differentiation, or that cells do not mature in vitro after D100.
