Clone 7010 was used to make chimera mice as described previously [66]. Removal of frt-Neo-frt and deletion of LoxP-exon2-LoxP was accomplished by crossing to mice expressing Tg-CMV-Flp and Tg-CMV-Cre to BptfFloxedNeo to create the BptfFloxed and BptfΔexon2 lines respectively. Individual recombinants were identified by Southern blotting and maintained by backcrossing to C57B6/CRL.
