A human GABAA subunit α2 cDNA (Hadingham et al. 1993a); GenBank accession number NM_000807) from GeneCopoeia (Germantown, MD) was subcloned into pcDNA3.1(+) (Invitrogen, Carlsbad, CA) for expression studies. The human β2 subunit cDNA ((Hadingham et al. 1993b); NM_000813) in pCDM8 was a kind gift from Peter Wingrove from Merck Laboratories. The human γ3 subunit cDNA (Hadingham et al. 1995); NM_033223) in pCMV6-XL5 was from Origene (Rockville, MD). The authenticity of all three subunits was verified by DNA sequencing before expression studies were conducted.
