Handling, sorting and analysis of small model organisms widely used in biology, Caenorhabditis elegans and Drosophila melanogaster, have been facilitated by developing special microfluidic devices [76]. Precise anesthesia-free positioning and immobilization of small model organisms for in vivo microscopy also greatly benefits from microfluidic devices. For example, neurobiologists studying small model organisms have found microfluidic devices useful for imaging neuronal transport of GFP-tagged cargos in living C. elegans and Drosophila larvae [77] and for studying synaptic transmission in neuromuscular junction in C. elegans [78]. Also, microfluidic device-assisted live animal studies of axonal injury have been performed in C. elegans [79] and in Drosophila larvae [50].
