After that, we followed up another four SNPs using a custom Illumina GoldenGate Genotyping Universal-32, 1536-plex micro-array assay (Illumina, San Diego, California). The majority of SNPs included in the custom array were selected for studies of other phenotypes. We followed up the top two most significant SNPs in the GWAS results of AAs and another two SNPs that map to obvious candidate loci and were among the top 10 most significant SNPs in the GWAS results of EAs. A total of 2553 subjects were genotyped and 2441 were retained after quality control. Among them, 505 did not have AIMs information and were grouped using self-reported population information.
