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Chunk #32 — Methods — Measurements and data analysis — Prevention of capitalization upon chance: Variable number reduction by creation of coherence factors

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A stable pattern of EEG spectral coherence distinguishes children with autism from neuro-typical controls - a large case control study.
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In order to facilitate subsequent statistical analysis, specifically in order to avoid capitalization on chance resulting from the use of too many variables, Principal Components Analysis (PCA) of the coherence data was employed as an objective technique to meaningfully reduce variable number [52]. The coherence data were first normalized (centered and shifted to have unit variance) so that eventual factors reflected deviations from the average. In order to avoid loss of sensitivity by a priori data limitation, an unrestricted form of PCA [53] was applied allowing all coherence variables per subject to enter analysis. By employment of an algorithm based upon singular value decomposition (SVD) [37,54], a data set of uncorrelated (orthogonal) principal components or factors [52,53] was developed in which the identification of a small number of factors following Varimax rotation [55] describe an acceptably large amount of variance [56]. Varimax rotation enhances factor contrast yielding higher loadings for fewer factors while retaining factor orthogonality. Although not the only PCA method applicable to large, asymmetrical matrices (4,416 variables by 1,034 cases as in the current study), SVD, which may