Deciduas used for sectioning were dissected from the uterus in PBS, washed once with PBS and immersed in 4% PFA buffered with PBS overnight at 4°C. Deciduas were then dehydrated in an ethanol series and imbedded in paraffin. Paraffin blocks were trimmed and sectioned and sections were stained with hematoxylin and eosin, TUNEL assay and phosphorylated H3 according to standard procedures [67].
