Transcription factors important for MGE specification were expressed at moderate to high levels (NKX2-1, ASCL1, DLX1, DLX5, LHX8 and LHX6)(Anderson et al., 1997a; Anderson et al., 1997b; Casarosa et al., 1999; Eisenstat et al., 1999; Flandin et al., 2011; Hansen et al., 2013). ZEB2, SOX6, ARX, and SATB1, were expressed at moderate levels from D54 on (Batista-Brito et al., 2009; Close et al., 2012; Colombo et al., 2007; McKinsey et al., 2013). The expression pattern of these transcription factors is reminiscent of the temporal progression of gene expression for MGE-like progenitors and interneurons.
