highly effective for introduction of SNPs (Kim et al. 2014; Liang et al. 2015; Lin et al. 2014; Niu et al. 2016; Richardson et al. 2016; Song et al. 2015). This obviates the need for any cloning or DNA manipulation, and all components can be purchased from commercial vendors. However, longer homology constructs have also been effectively used to introduce point mutations in other published reports (Wang et al. 2017; Yusa 2013).
