Our targeted sequencing of CHRNA5 in approximately 3000 nicotine dependent cases and non-dependent controls builds upon previous exome chip and sequencing studies of other smoking-related measures.22,28-30 Sequencing a large, diverse, unrelated sample (n=2820) enabled us to identify many more coding variants of high quality (n=26), including 9 novel variants, and demonstrate association with nicotine dependence.
