spontaneously differentiated EBs and significant variation in erythroid differentiation potential between iPSC lines derived from two healthy donors, regardless of the cell type of origin. Using GSEA we were able to associate the low erythroid-forming potential of the healthy donor to genes previously indicated in DBA. Moreover, the differential expression of these genes in different donors at the iPSC stage was, at least in part, maintained through differentiation to EBs, providing further evidence in support of the donor-related differences affecting the differentiation potential of iPSC lines.
