al., 2013; Mandegar et al., 2016; Ordovas et al., 2015) and demonstrate that recently described systems for inducible CRISPR/Cas9 (González et al., 2014; Mandegar et al., 2016) are unlikely to work in a diversity of hPSC-derived cell types. For this reason, we explored the possibility of developing an alternative and improved method by combining a constitutively expressed CAG promoter-driven Cas9 with an inducible gRNA cassette based on that developed for inducible shRNA expression in sOPTiKD (Fig. 7A,B).
