NCBI provides several tools that assist in identifying coding sequences in genomic DNA. The Open Reading Frame (ORF) Finder (www.ncbi.nlm.nih.gov/projects/gorf/) performs a six-frame translation of a nucleotide sequence and returns the location of each ORF within a specified size range. Spidey aligns a set of eukaryotic mRNA sequences to a single genomic sequence taking into account predicted splice sites and using one of four splice-site models (Vertebrate, Drosophila, C. elegans, Plant).
