To test the hypothesis that AUD PRS affects cellular mechanisms, we prepared 18 iPSC lines from lymphocytes and lymphoblastoid cells from participants collected by the COGA (41–43) based on their AUD PRS (12). Eight participants (five men and three women) had AUD and a PRS > 75 percentile (high-PRS, seven of which were above 90 percentile), and 10 (five men, five women) were unaffected participants (no AUD) a PRS < 25 percentile (low-PRS, nine below were 10 percentile) (table S1). The pluripotency of these iPSC lines was validated with immunohistochemical (IHC) staining with pluripotency markers such as Oct4, SOX2, and TRA-160 (fig. S1). Furthermore, we also analyzed chromosomal aberrations by allelic bias using RNA sequencing (RNA-seq) data, i.e., expression-based single nucleotide polymorphism (e-SNP)–karyotyping (44) and G-band karyotyping. We found no significant chromosomal aberrations in these iPSC lines (P < 0.001; figs. S2 and S3). All COGA iPSC lines can be requested from National Institute on Alcohol Abuse and (NIAAA) (https://cogastudy.org/resources-for-researchers/).
