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Chunk #5 — Methods — Analysis of Locomotion (crawling)

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Ethanol metabolism and osmolarity modify behavioral responses to ethanol in C. elegans.
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Age-matched 55 hour-old adult animals reared at 20°C were used. Locomotion on plates was assayed as described (Davies et al., 2003). Ten worms for each strain were tested. Two minute movies were recorded, and movies were analyzed using ImagePro Plus Version 6 (Media Cybernetics, Bethesda, MD, U.S.A.). To account for differences in basal speeds, we calculated a relative speed (ethanol-treated average speed / untreated average speed × 100).