Scientific, Rockwood, TN) with 7 µl 0.05% n-propanol, as an external pippeting standard. Samples, in duplicate, were heated to 65°C for 20 min, agitated for 30 sec, and allowed to settle for 1 min prior to pressurizing for headspace extraction into a 2 ml, depolarized loop (Tekmar Control Systems). Samples were immediately passed through a 220°C deactivated, glass lined inlet (Hewlett-Packard) and subjected to gas chromatography (He, 5 kPa) on a megabore 30 m, 1 µm film innowax column (Agilent Technologies) at a 45°C isotherm and quantified via flame ionization at 310°C in a deactivated jet and detector (Agilent Technologies). The column was purged after each sample by holding at 210°C for 1.5 min prior to cooling to 45°C over 5 min. The alcohol area under the curve (AUC) was divided by the external n-propanol standard AUC and compared to known standards from 300 mM–0.003 mM (run in duplicate, r2 typically = 1.00) that were freshly prepared for every run.
