as, CCDC71, NCKIPSD, and MST1R. O-6-Methylguanine-DNA Methyltransferase (MGMT) was the most significant gene outside of chromosome 3 (ZTWAS = −5.33, p = 9.88 × 10−8 – caudate basal ganglia), followed by CDK11B on chromosome 1, and the long non-coding RNA (lncRNA) LINC00324 on chromosome 17. Previous analysis, differing in methodology and samples, found an association between SUOX expression and AN which was not replicated in the present study (Baird et al., 2021; Chatzinakos et al., 2020). Using a more lenient FDR approach for multiple-testing correction revealed more association signals (273 signals, 104 unique genes, online Supplementary Table S1). Additionally, we replicated 18 of the 36 significant associations from a previously published AN PrediXcan analysis, including WDR6 and their most significant association DALRD3. We posit that any signals not replicated would be a function of FUSION only using cis-heritable genes, differences in GReX construction between the methods, and our study only including brain and blood tissues.
