Chunk #40 — MATERIALS AND METHODS — Transfections

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Inhibition of TBC1D5 activates Rab7a and can enhance the function of the retromer cargo-selective complex.

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Transient transfections of the HeLa cells were performed using polyethylenimine (PEI) as described in Harbour et al. (2010). The siRNA knockdowns were performed using siRNA oligos purchased from Dharmacon following a protocol also described in Zavodszky et al. (2014).