Chunk #8 — Materials and methods — ASE analysis

Source

Allele-specific expression and high-throughput reporter assay reveal functional genetic variants associated with alcohol use disorders.

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GATK ASEReadCounter was used to obtain reference and alternative allele counts at the exonic SNPs [19]. We only analyzed SNPs that were heterozygous in at least five control and five AUD groups, and that also had more than 10 reads, to ensure that samples with sufficient reliable read counts were used for analysis.