Chunk #42 — Experimental Procedures — Chromatin Immunoprecipitation (ChIP)

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Simple combinations of lineage-determining transcription factors prime cis-regulatory elements required for macrophage and B cell identities.

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ChIP was performed with 10–20×106 cells essentially as described (Metivier et al., 2003). Antibodies used: PU.1 (sc-352), C/EBPα (sc-61), C/EBPβ (sc-150), Oct-2 (sc-233) and pan-LXR (sc-1000) (Santa Cruz Biotech), H3K4me1 (ab8895) and H3K4me3 (ab8580) (Abcam) or LXRβ (PP-K8917-00, Perseus Proteomics).