Compared with previous UMI-based ((Zeisel et al., 2015); detects 1.8–4.7K genes/cell) and non-UMI based method ((Tasic et al., 2016), detects ~7.2K genes/cell), our linear amplification with 10bp UMIs improved gene detection and quantification (~9K genes/cell; Figure S1H). Compared with Dropseq which allows vast throughput at lower resolution (Shekhar et al., 2016), our complementary approach achieved more comprehensive and quantitative transcriptome measurement of targeted cell populations.
