Chunk #33 — Methods — Subjects and blood samples

Source

Variability of gene expression profiles in human blood and lymphoblastoid cell lines.

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Only two people at a time were sampled on any one day for logistical reasons. After blood draw standard protocols were followed for cell isolation, transformation or RNA extraction. With the exception of the PAXgene samples all RNA was isolated using TRI™ reagent (SIGMA) and resuspended in RNase free water.