Chunk #16 — Materials and Methods — Electrophysiology

Source

Evidence that increased Kcnj6 gene dose is necessary for deficits in behavior and dentate gyrus synaptic plasticity in the Ts65Dn mouse model of Down syndrome.

Embedded

yes

Text

with three trains of stimuli (1 sec at 100 Hz; 5 min between the trains). Short-term potentiation (STP) and LTP were evaluated at 1–15 min and 30–60 min after the tetanus respectively. Fluoxetine hydrochloride (Sigma-Aldrich, cat. # F-132) was dissolved in distilled water to a concentration of 2 mM, aliquoted, and stored at −30 °C. Fluoxetine was bath applied, at a final concentration of 10 μM, for 15 min before and 15 min after the tetanus.