enriched cell lysates detects ZBTB16 expression increased ASC SUMOylation (Fig. 5d). This ZBTB16-dependent increased ASC SUMOylation was confirmed for the endogenous proteins by immunoblotting peptides enriched with an anti-ASC or control anti-IgG antibodies from WT and Zbtb16- /- BMDMs with an anti-SUMO1 antibody (Fig. 5e). Consistent with these data, ASC and SUMO1 were detected to colocalize to a greater extent in the nucleus of WT compared to Zbtb16-/- BMDMs after stimulation with LPS and nigericin as measured by immunofluorescence (Fig. 5f). Notably, Zbtb16 did not alter the expression of the components of the SUMO pathway (Supplementary Fig. 5b, c).
