dopaminergic neurons initiate the expression of midbrain and dopaminergic markers, such as LMX1A, FOXA2 and tyrosine hydroxylase (TH). These markers are commonly used to assess the efficiency of neuronal differentiation. We compared neurons plated on micropatterned substrates with those grown on conventional unpatterned PDL/laminin-coated glass coverslips on day 70 of differentiation and were able to confirm that similar numbers of neurons plated on either systems stained positive for TH expression (Figure 2D). These results indicate that micropatterned culture conditions did not alter the maturation process in comparison with conventionally used unpatterned PDL/laminin-coated glass coverslip surfaces for iPSC-derived neuronal cultures.
